Research groups
Neuropathological Research Group
Endre Pál (associate professor) E-mail: pal.endre@pte.hu
Tel.: +36 (72) 535-916, +36 (72) 536-000 (ext: 31849)
Research interest

Muscle diseases

-        Muscle histology. Muscular Dystrophies, Mitochondrial Disorders, Inflammatory myopathies (myositis).

-        The pathomechanism of myotonic dystrophy types 1 and 2 (DM1-2) is investigated by quantitative histological methods, immunofluorescence and in situ hybridization.

-        Investigation of the pathomechanism of muscle atrophy by immuno-histochemical, molecular biological methods.

-        Investigation of mitochondrial damage in muscle diseases by enzyme histochemical and molecular biological methods.

Peripheral nervous diseases.

-        Clinical diagnosis of inflammatory and hereditary neuropathies.

Skin biopsy: diagnosis of thin-fiber neuropathy by immunohistochemistry.

Biomarkers in neuromuscular and neurodegenerative diseases.

-        Serum and CSF biomarkers (FGF21 and GDF15)

Neurodegenerative disorders

-        Alpha-synuclein in peripheral tissues

-        Alpha synuclein and p-Tau in serum and CSF samples

-        SNCA SNP investigation in Parkinson disease

-        Serum biomarkers of the oxidative stress in Parkinson disease

-        Measurement of neurofilament light chain (NFL) in CSF and sera in subtypes of patients with amyotrophic lateralsclerosis (ALS)

Clinical relevance

Differential diagnosis of hereditary muscle diseases, orientation of genetic tests

Establishment of registers for muscle diseases

Histological and molecular diagnostics of myotonic dystrophy type 1 and 2.

Investigation of biomarkers in neuromuscular and neurodegenerative disorders: (fibroblast growth factor 21 (FGF21), growth differentiation factor 15 (GDF15), neurofilament light chain (NFL), synuclein-alpha, PGP 9.5) in histological and blood samples to (1) improve the diagnostic safety, (2) definition of subgroups regarding to the disease severity and progression. 

Tools
ELISA appatátus
mikrotóm, kriosztát
Western-blot apparatus
Methods
ELISA
Enzimhisztokémia (ATP-aze különböző pH-n, savas foszfatáz, alkalikus foszfatáz, foszforiláz, myoadenilát, NADH, szukcinát dehidrogenáz (SDH), citokróm-c oxidáz (COX))
IF vizsgálat
Rutin hisztokémia (HE, PAS, Oil red, Gömöri, congó)
Laboratory members
Emese Lovadi