The research program of the Group summarizes our multiple neurophysiological experiments to functionally characterize the glucose-monitoring (GM) neuronal network substantial in the central regulation of feeding, body weight, and metabolism. Extracellular single neuron recording, neurochemical-biochemical and behavioral investigations are planned in the laboratory rat. The target areas of the experiments are the limbic forebrain structures, well known for their distinguished regulatory importance, in which the homeostatic role of the GM neurons is to be elucidated.
Successful accomplishment of the program serves the better understanding of the central regulation of feeding and metabolism and that of diseases (obesity, diabetes mellitus, etc.) developing after pathological alterations of these functions and causing escalating social and financial problems worldwide. In addition to elucidate so far unknown regulatory processes and pathological mechanisms, it is highly hoped that our research approach enables us to identify new molecular drug-targets in the limbic forebrain and leads to the development of novel therapeutic approaches as well.
Cerebral microinjection set-up Cold chemistry photometric plasma metabolite measuring equipment Extracellular single neuron recording set-up Taste reactivity measuring set-up
Brain microinjection technique in freely moving animal Evaluation of taste information processing: 1) conditioned taste avoidance and aversion tests; 2) taste reactivity tests Metabolic measurements: 1) determination of plasma metabolite concentrations; 2) glucose tolerance test Single neuron recording – the multibarreled microelectrophoretic technique
Multiple functional attributes of glucose-monitoring neurons in the medial orbitofrontal (ventrolateral prefrontal) cortex.
Szabo I., Hormay E., Csetenyi B., Nagy B., Lenard L., Karadi Z.
Neuroscience and Biobehavioral Reviews (2018/85: 44-53.)
Food and water intake, body temperature and metabolic consequences of interleukin-1β microinjection into the cingulate cortex of the rat.
Csetenyi B., Hormay E., Szabo I., Takacs G., Nagy B., Laszlo K., Karadi Z.
Behavioural Brain Research (2017/331: 115-122.)
Noradrenaline and acetylcholine responsiveness of glucose-monitoring and glucose-insensitive neurons in the mediodorsal prefrontal cortex.
Nagy B., Szabo I., Csetenyi B., Hormay E., Papp Sz., Keresztes D., Karadi Z.
Brain Research (2014/1543: 159-164.)
Glucose-monitoring neurons in the mediodorsal prefrontal cortex.
Nagy B., Szabo I., Papp Sz., Takacs G., Szalay Cs., Karadi Z.
Brain Research (2012/1444: 38-44.)
Taste reactivity alterations after streptozotocin microinjection into the mediodorsal prefrontal cortex.
Nagy B., Takacs G., Szabo I., Lenard L., Karadi Z
Behavioural Brain Research (2012/234(2): 228-232.)
EFOP-3.6.1-2016-00004. Investigation of the peripheral and central regulation of metabolism by bioimpedance measurements, human behavioral physiological and complex neurophyisological methods. 2016-2020.