In the past 15 years we have been studying the neurodegeneration of the retina in different experimental pathological conditions (diabetic retinopathy, retinal ischemia, excitotoxic retinal damage) in rats and mice. We have applied different neuroprotective treatments to decrease the damege and observe the cellular and molecular mechanisms underlying the tissue injury and retinoprotection. We have put particular emphasis on PACAP-induced neuroprotection.
Recently we have also started to investigate the effect of PACAP KO on retinal aging in mice, as well as the normal aging process in rats and mice. Along with the aging process, we also started to explore the role of non-conventional signaling pathways in postnatal retina development. We are interested mostly in the microRNA-indiced changes of gene expression. We are developing tools to visualize miRNA expression together with cellular and synaptic markers of retinal neuron.
We wish to provide preclinikal models for several retinal pathologies (e.g. diabetic retinopathy, glaucoma) and provide full description of cellular and molecular events in model animals. These studies may help to understand the biological backgroung, plan therapies and diagnostic tolls as well as to identify potential drug targets.
Elektronmikroszkópia microarray PCR Rutin hisztológiai eljárások Western blot
Accelerated retinal aging in PACAP knock-out mice.
Kovacs-Valasek A., Szabadfi K., Denes V., Szalontai B., Tamas A., Kiss P., Szabo A., Jr. Setalo G., Reglodi D., Gabriel R.
Neuroscience (2017/348: 1-10.)
Ectopic transgene expression in the retina of four transgenic mouse lines.
Gabriel R., Erdelyi F., Szabo G., Lawrence J.J., Wilhelm M.
Brain Structure and Function (2016/221(7): 3729-3741.)
Pituitary adenylate cyclase activating polypeptide, a new potential therapeutic agent for diabetic retinopathy in rats: focus on the vertical information processing pathway.
Szabadfi K., Reglodi D., Szabo A., Szalontai B., Valasek A., Jr. Setalo Gy., Kiss P., Tamas A., Wilhelm M., Gabriel R.
Neurotoxicity Research (2016/29(3): 432-446.)
Pituitary adenylate cyclase-activating peptide (PACAP), a novel secretagogue, regulates secreted morphogens in newborn rat retina.
Lakk M., Denes V., Kovacs K., Hideg O., Szabo B.F., Gabriel R.
Investigative Ophthalmology and Visual Science (2017/58(1): 565-572.)
Retinal aging in the diurnal Chilean rodent (Octodon degus): Histological, ultrastructural and neurochemical alterations of the vertical information processing pathway.
Szabadfi K., Estrada C., Fernandez-Villalba E., Tarragon E., Setalo G., Izura V., Reglodi D., Tamas A., Gabriel R., Herrero M.T.
Frontiers in Cellular Neuroscience (2015/9(APR): 126.)
KTIA - NAP 1. és NAP2.